Skin Irritation Test (In Vitro)

Skin Irritation Test

These days, a person comes into contact with products containing different chemicals such as cosmetics, detergents, soaps, biocidal products. The reaction that each person will develop depending on a product they come into contact with may be different. A product goes through many tests and is analyzed during the time it takes to reach the consumer. "Irritation Tests" are among the most performed tests.

It covers the evaluation of medical devices, cosmetic products, or drug formulations for their potential for irritation and skin sensitization. This method is based on in vitro tissue culture testing as an alternative method without the use of experimental animals.

In this method, a reconstructed three-dimensional human skin model is used in the laboratory. Sample, Positive Control, and Negative control groups are tested simultaneously. Cell viability in tissues after the application is measured in a spectrophotometer. A ratio of the number of viable cells in the sample to the number of viable cells in the negative control >50% indicates that the sample is not irritant.

While performing the irritation tests of pharmaceuticals, chemicals, and medical devices, certain standards are followed and these tests are carried out by accredited laboratories. As a result, irritation tests are very important for human health and a product that will be released on the market must be tested by accredited and authorized laboratories.